@Article{AntoniniKoReOwMaMa:2017:EfTiSu,
author = "Antonini, Leonardo Marasca and Kothe, Vin{\'{\i}}cius and
Reilly, Gwendolen C. and Owen, Robert and Marcuzzo, Jossano
Saldanha and Malfatti, C{\'e}lia de Fraga",
affiliation = "{Universidade Federal do Rio Grande do Sul (UFRGS)} and
{Universidade Federal do Rio Grande do Sul (UFRGS)} and
{University of Sheffield} and {University of Sheffield} and
{Instituto Nacional de Pesquisas Espaciais (INPE)} and
{Universidade Federal do Rio Grande do Sul (UFRGS)}",
title = "Effect of Ti6Al4V surface morphology on the osteogenic
differentiation of human embryonic stem cells",
journal = "Journal of Materials Research",
year = "2017",
volume = "32",
number = "20",
pages = "3811--3821",
month = "Oct.",
abstract = "Ti6Al4V alloys usually need to undergo some kind of surface
treatment to enable good bone growth and implant integration. In
this work, three treatments that modify the titanium alloy surface
were studied with the aim of promoting osteogenic differentiation
of human-embryonic-stem-cell-derived mesenchymal progenitors
(hESC-MPs). The surface treatments used were mechanical polishing
and electropolishing for 4 or 12 min in an H2SO4/HF/glycerine
solution. The samples were characterised by atomic force
microscopy, profilometry, X-ray photoelectron spectroscopy, and
wettability. Samples were seeded with hESC-MPs in osteogenic
media, and the cell number and alkaline phosphatase activity were
measured. The electropolishing surface treatments influenced the
nanometric morphology and wettability. However, the
electropolished surfaces contributed in the same way as the
mechanically polished surface to osteogenic differentiation,
indicating that differentiation was strongly influenced by
microroughness, which did not differ among the treatments used in
the present work.",
doi = "10.1557/jmr.2017.392",
url = "http://dx.doi.org/10.1557/jmr.2017.392",
issn = "0884-2914",
language = "en",
targetfile = "antonini_effect.pdf",
urlaccessdate = "28 abr. 2024"
}